human tongue squamous cell carcinoma cell line cal 27  (ATCC)

Journal: Pharmaceuticals (Basel, Switzerland)

Article Title: New Evidence for Cotinus coggygria Scop. Extracts Application in Gastrointestinal Ailments.

doi: 10.3390/ph18010098

Figure Lengend Snippet: Figure 1. Cytotoxic effect of C. coggygria extracts against human squamous cell carcinoma and human colon cancer cell lines. (A) Effects of C. coggygria extracts on CAL27 (A), FaDu (B), SW480 (C), HCT116 (D) and MRC-5 (E) cell viability after 48 and 72 h, determined by MTT assay. The results represent the mean ± SD of three independent experiments (each performed in triplicates).

Article Snippet: Human squamous cell carcinoma cell lines CAL27 (tongue) and FaDu (pharynx), human colon cancer cell lines (SW480 and HCT116) and human fibroblast cell line MRC-5 were purchased from American Type Culture Collection.

Techniques: MTT Assay

Journal: bioRxiv

Article Title: An extended and apical supracellular actin network interconnects squamous carcinoma cells

doi: 10.1101/2025.01.10.632450

Figure Lengend Snippet: (A-E) Representative bright-field images of the gaps between both cell sheets of HaCaT (A) , HSC-5 (B) , CAL27 (C) , A549 (D) and U2OS (E) cell sheets at 0, 24, 48 and 72h of migration after removing the insert. The yellow lines represent the contour of the gaps not covered by the cells (wound area). Scale bar = 200 µm. (F-J) Representative bright-field images of the outside periphery of both HaCaT (F) , HSC-5 (G) , CAL27 (H) , A549 (I) and U2OS (J) cell sheets at 0, 24, 48 and 72h of migration after removing the insert. The yellow arrowheads point towards multicellular structures called fingers. Scale bar = 200 µm. (K) Wound area after 0, 24, 48 and 72h of HaCaT, HSC-5, CAL27, A549 and U2OS cell migration. For each condition, 3 to 8 pictures per experiment obtained in 3 independent experiments were analysed. All the data are presented as mean ± s.d. and tested by Kruskal-Wallis (ns: not significant; *: p<0,05; **: p<0,01; ****: p<0,0001).

Article Snippet: Human skin squamous carcinoma A431 cell line (#CRL-1555), human tongue squamous carcinoma CAL27 cell line (#CRL-2095) and immortalized human embryonic kidney HEK-293T cell line (#CRL-3216) were purchased from ATCC.

Techniques: Migration

Journal: European journal of medicinal chemistry

Article Title: Multicomponent syntheses enable the discovery of novel quisinostat-derived chemotypes as histone deacetylase inhibitors.

doi: 10.1016/j.ejmech.2024.117045

Figure Lengend Snippet: Fig. 6. Hyperacetylation of α-tubulin and histone H3 was determined as a surrogate of cellular HDAC inhibition. Cal27 cells were treated at IC50 concentrations of the MTT assays with test compounds for 24 h. Acetylation was analyzed by immunoblot analysis using antibodies against acetyl-α-tubulin and acetyl-histone H3. GAPDH was used as loading control. The shown Western blot is a representative blot of two individual experiments.

Article Snippet: The human tongue squamous cell carcinoma cell line Cal27 was obtained from the German Collection of Microorganisms and Cell Cultures (DSMZ, Hannover, Germany).

Techniques: Inhibition, Western Blot, Control